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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Objectives |
|---|---|
| Topic 1: Microbiology | - Clinical microbiological techniques
|
| Topic 2: Hematology | - Blood cell analysis and disorders
|
| Topic 3: Urinalysis and Body Fluids | - Fluid analysis and interpretation
|
| Topic 4: Clinical Chemistry | - Metabolic and analytical testing
|
| Topic 5: Blood Banking / Immunohematology | - Blood group systems and antibody identification
|
| Topic 6: Laboratory Operations | - Quality and safety in the clinical lab
|
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
G6PD deficiency causes an increased suseptibility to the oxidation of hemoglobin, which in turn, causes the precipitation of hemoglobin inside of the red blood cell.
Which one of the following conditions is associated with glucose-6-phosphate dehydrogenase (G6PD) deficiency?
- A. Precipitation of hemoglobin
- B. Microcytic red cells
- C. Faulty heme synthesis
- D. Hemoglobins with low oxygen affinities
Correct Answer: A 🗳️
Western blot analysis is frequently utilized as the confirmatory method of HIV detection.
Which of the following assays is routinely used for confirmation of HIV infections:
- A. Southern blot
- B. In-situ hybridization
- C. Radioimmunoassay
- D. Western blot
Correct Answer: D 🗳️
Gating sets up boundaries around a subset of data points to segregate those events for analysis.
Gating the proper cell population is crucial for flow cytometric analysis. The CD marker information presented on the histogram data is only representative of the cells inside the gate.
When analyzing the raw data of a sample on the flow cytometer, CD marker information on the histograms represents data inside the _____ population.
- A. Non-gated
- B. Gated
Correct Answer: B 🗳️
Bone marrow biopsies are helpful diagnostic tools in:
Evaluation of anemia and thrombocytopenia
Evaluation of cytopenias
Diagnosing leukemias
Can be part of evaluation for FUO
Bone marrow biopsies are NOT used as screens for hematologic issues during routine check-ups.
Hematology
In which of the following situations would a bone marrow aspirate and biopsy possibly be used as an aid for diagnosis? (Select all that apply)
- A. Screening for hematologic issues during routine check-ups
- B. Evaluation of fever of unknown origin (FUO)
- C. Diagnosis of leukemias
- D. Evaluation of anemia and thrombocytopenia
- E. Evaluation of cytopenias
Correct Answer: B,D 🗳️
RAST tests, or Radioallergosorbent tests, are used to screen for an allergy to a specific substance or substances if a person presents with allergy-like symptoms.
The assay which is most helpful in identifying specific allergens is:
- A. RAST
- B. Complement fixation
- C. RIA
- D. C-reactive proteins
Correct Answer: A 🗳️

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