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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Laboratory Operations | 5–10% | - Instrumentation and laboratory mathematics - Quality assessment and safety |
| Immunology | 5–10% | - Serologic procedures and immune diseases - Principles of immunology |
| Blood Banking | 15–20% | - Transfusion practice and testing - Blood products and components - Blood group systems and immunology |
| Urinalysis and Other Body Fluids | 5–10% | - Physical and chemical testing - Microscopic analysis and disease correlation |
| Hematology | 20–25% | - Physiology and disease states - Laboratory testing and hemostasis |
| Microbiology | 15–20% | - Bacteriology and mycology procedures - Virology and parasitology testing |
| Chemistry | 20–25% | - Carbohydrates, lipids, and enzymes - Electrolytes, acid-base, and toxicology |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
High-sensitivity C-reactive protein (hs-CRP) is used as an aid in the diagnosis and treatment of cardiovascular disease (CVD). At low levels, it can detect those at risk for cardiac heart disease. At high levels in those with no history of heart disease, it indicates a high risk for acute myocardial infarction (AMI), stroke, or peripheral vascular disease. For patients with acute coronary syndrome (ACS) or stable coronary disease, hs-CRP is used to predict future coronary events.
Ranges of hs-CRP in prediction of risk for CVD are:
<1.0 mg/L Low CVD risk
1.0-3.0 mg/L Average risk for CVD
>3.0 mg/L High risk for future CVD
If results are >10.0 mg/L, the patient should be evaluated for an acute inflammatory condition.
Chemistry
A high-sensitivity C-reactive protein (hs-CRP) test result of 5 mg/L (normal = < 1 mg/L) may indicate which of the following?
- A. Low risk for cardiovascular disease (CVD)
- B. High risk for future CVD
- C. Acute inflammatory condition
Correct Answer: B 🗳️
Hb S migrates with D in alkaline and alone in acid electrophoresis.
The gel electrophoresis pattern for hemoglobin S shows which of the following migration patterns?
- A. Hb S migrates with D in alkaline and A in acid electrophoresis.
- B. Hb S migrates alone in alkaline and D in acid electrophoresis.
- C. Hb S migrates with A in alkaline and D in acid electrophoresis.
- D. Hb S migrates with D in alkaline and alone in acid electrophoresis.
Correct Answer: D 🗳️
Normocytic- G6PD deficiency, Malaria
Microcytic- Iron deficiency, Thalassemia
Macrocytic- Chronic Liver Disease, Vitamin B12 deficiency
Hematology
Match the disease conditions with appropriate red cell size classification
1. G6PD deficiency, Malaria
2. Iron deficiency, Thalassemia
3. Chronic Liver Disease, Vitamin B12 deficiency
- A. Microcytic
- B. Normocytic
- C. Macrocytic
Correct Answer: A,B,C 🗳️
Alkaline phosphatase, or ALP, is present in kidneys, liver, intestines, bone, and the placenta. The liver makes the largest amount of ALP. Some of the conditions associated with increased levels of ALP include: damaged liver cells, rapid bone growth (during puberty), bone diseases, or a disease that affects how much calcium is in the blood (hyperparathyroidism), and vitamin D deficiency.
Chemistry
Isoenzymes of alkaline phosphatase occur in:
- A. Liver, brain, spleen, intestines
- B. Kidney, bone, intestines, liver
- C. Bone, brain, liver, pancreas
- D. Brain, kidney, intestines, bone
Correct Answer: B 🗳️
Hives and rash usually indicate an allergic reaction. Hematuria is due to a variety of causes. Fever and chills usually indicate a febrile reaction. Positive DAT due to conditions other than sensitization to red cell alloantigens is not uncommon. Therefore a positive DAT in the posttransfusion specimen with a negative DAT in the pretransfusion specimen is more likely to indicate alloimmunization.
The most definite indication that a patient has been sensitized to a specific red cell antigen is:
- A. Hemoglobinuria
- B. A positive posttransfusion DAT in a patient with a previously positive DAT
- C. A positive posttransfusion DAT in a patient with a previously negative DAT
- D. Hives and rash
Correct Answer: C 🗳️
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