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American Society of Microbiology ABMM Sample Questions:

1. A clinical microbiology laboratory is performing antimicrobial susceptibility testing using the broth microdilution method. The minimum bactericidal concentration (MBC) is determined by:

A) The lowest concentration of antibiotic that inhibits visible growth after 24 hours of incubation.
B) Subculturing the clear wells from the MIC assay onto antibiotic-free media and determining the lowest concentration that results in no bacterial growth.
C) The highest concentration of antibiotic that allows visible growth after 24 hours of incubation.
D) Comparing the zone of inhibition around antibiotic disks to standardized tables.

2. A research laboratory is investigating the mechanism of action of a novel antiviral compound. In vitro studies demonstrate that the compound effectively inhibits the replication of a specific enveloped RNA virus. Further analysis reveals that the compound does not interfere with viral entry, genome replication, or protein synthesis. However, electron microscopy of infected cells treated with the compound shows an accumulation of viral glycoproteins within the endoplasmic reticulum and Golgi apparatus, with a significant reduction in the release of infectious virions. The MOST likely target of this antiviral compound is:

A) Viral protease responsible for polyprotein cleavage
B) Viral glycoprotein trafficking and maturation
C) Viral RNA-dependent RNA polymerase
D) Host cell membrane fusion machinery

3. A patient with a prosthetic joint develops a chronic infection. Multiple synovial fluid cultures grow a Gram- positive coccus that forms a biofilm and is resistant to multiple antibiotics, including methicillin and gentamicin. The MOST likely organism is:

A) Staphylococcus epidermidis
B) Staphylococcus aureus
C) Streptococcus agalactiae
D) Enterococcus faecium

4. A patient with HIV infection and a CD4+ T-cell count below 50 cells/µL develops a progressive neurological illness characterized by confusion, motor deficits, and seizures. Brain MRI reveals multiple white matter lesions without mass effect. PCR of the CSF is positive for JC virus. Thepathogenesis of this condition, progressive multifocal leukoencephalopathy (PML), involves:

A) Inflammatory demyelination triggered by JC virus infection of astrocytes.
B) Opportunistic infection by JC virus due to impaired cell-mediated immunity.
C) Lytic infection of oligodendrocytes by JC virus, leading to demyelination.
D) Direct cytopathic effect of JC virus on neurons.

5. An immunocompromised patient develops a disseminated fungal infection. Tissue biopsy reveals small (2-4
µm) yeast cells within macrophages. The patient has a history of living in the Ohio and Mississippi River valleys. The MOST likely causative agent is:

A) Blastomyces dermatitidis
B) Histoplasma capsulatum
C) Paracoccidioides brasiliensis
D) Penicillium marneffei

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